Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition.

ABSTRACT: A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.e

SUBMITTER: Alberti A 

PROVIDER: S-EPMC5538240 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature