DNA replication timing during development anticipates transcriptional programs and parallels enhancer activation.

ABSTRACT: In dividing cells, DNA replication occurs in a precise order, but many questions remain regarding the mechanisms of replication timing establishment and regulation. We now have generated genome-wide, high-resolution replication timing maps throughout zebrafish development. Unexpectedly, in the rapid cell cycles preceding the midblastula transition, a defined timing program was present that predicted the initial wave of zygotic transcription. Replication timing was thereafter progressively and continuously remodeled across the majority of the genome, and epigenetic changes involved in enhancer activation frequently paralleled developmental changes in replication timing. The long arm of Chromosome 4 underwent a dramatic developmentally regulated switch to late replication during gastrulation

SUBMITTER: Siefert JC 

PROVIDER: S-EPMC5538556 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature