Structural and functional analysis of the GABARAP interaction motif (GIM).

ABSTRACT: Through the canonical LC3 interaction motif (LIR), [W/F/Y]-X₁-X₂-[I/L/V], protein complexes are recruited to autophagosomes to perform their functions as either autophagy adaptors or receptors. How these adaptors/receptors selectively interact with either LC3 or GABARAP families remains unclear. Herein, we determine the range of selectivity of 30 known core LIR motifs towards individual LC3s and GABARAPs. From these, we define a G ABARAP I nteraction M otif (GIM) sequence ([W/F]-[V/I]-X₂-V) that the adaptor protein PLEKHM1 tightly conforms to. Using biophysical and structural approaches, we show that the PLEKHM1-LIR is indeed 11-fold more specific for GABARAP than LC3B. Selective mutation of the X₁ and X<

SUBMITTER: Rogov VV 

PROVIDER: S-EPMC5538626 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature