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Heritable genetic transformation of Strongyloides stercoralis by microinjection of plasmid DNA constructs into the male germline.

ABSTRACT: Heretofore, transgenesis in the parasitic nematode genus Strongyloides has relied on microinjecting transgene constructs into gonadal syncytia of free-living females. We now report transgenesis in Strongyloides stercoralis by microinjecting constructs into the syncytial testes of free-living males. Crosses of individual males microinjected with a construct encoding GFP with cohorts of 12 non-injected females produced a mean of 7.28±2.09 transgenic progeny. Progeny of males and females microinjected with distinct reporter constructs comprised 2.6%±0.7% of individuals expressing both paternal and maternal transgenes. Implications of this finding for deployment of CRISPR/Cas9 mutagenesis in Strongyloides spp. are discussed.

SUBMITTER: Shao H

PROVIDER: S-EPMC5538930 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Heritable genetic transformation of Strongyloides stercoralis by microinjection of plasmid DNA constructs into the male germline.

Shao Hongguang H Li Xinshe X Lok James B JB

International journal for parasitology 20170601 9

Heretofore, transgenesis in the parasitic nematode genus Strongyloides has relied on microinjecting transgene constructs into gonadal syncytia of free-living females. We now report transgenesis in Strongyloides stercoralis by microinjecting constructs into the syncytial testes of free-living males. Crosses of individual males microinjected with a construct encoding GFP with cohorts of 12 non-injected females produced a mean of 7.28±2.09 transgenic progeny. Progeny of males and females microinjec ...[more]

PMID: 28577882

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