High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing.

ABSTRACT: Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (10⁷ cells mL^-1) and a high droplet resolution of 1 nL. High-resolution 3D geometries were printed with features of ≤200 μm; these included an arborised cel

SUBMITTER: Graham AD 

PROVIDER: S-EPMC5539110 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature