Spatiotemporal Variation and Networks in the Mycobiome of the Wheat Canopy.

ABSTRACT: The phyllosphere is an important habitat for a diverse microbiome and an important entry point for many pathogens. Factors that shape the phyllosphere microbiome and also the co-existence among members and how they affect disease development are largely understudied. In this study we examined the wheat mycobiome by using metabarcoding of the fungal ITS1 region. Leaf samples were taken from four cultivars grown at two locations in Denmark. Samples were taken from the three uppermost leaves and at three growth stages to better understand spatiotemporal variation of the mycobiome. Analysis of read abundances showed that geographical location had a major effect in shaping the mycobiome in the total dataset, but also leaf position, growth stage and cultivar were important drivers of fungal comm

SUBMITTER: Sapkota R 

PROVIDER: S-EPMC5539183 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature