Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study.

ABSTRACT:

Background

Bull fertility is the degree of sperm's ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with well-established in vitro systems are available for bovine. The objective of this original study was to ascertain evolutionary diversification and expression dynamics of Testis Specific Histone 2B (TH2B) in sperm from Holstein bulls with different fertility scores.

Methods

The intensity of TH2B was determined by using flow cytometry in sperm from 13 high and 13 low fertility bulls. Expr

SUBMITTER: Kutchy NA 

PROVIDER: S-EPMC5539985 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature