Development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens.

ABSTRACT:

Aims

To develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens.

Methods and results

The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterococcus faecalis. All five RPAs required reaction times of under 12 min to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with high concentrations of nontarget bacterial genomic DNA. In a 50-sample retrospective clinical study, the five-RPA assay panel was found to have a specificity of 100% (95% CI, 78-100%) and a sensitivity of 89% (95% CI, 75-96%) for UTI detection.

Conclusions

SUBMITTER: Raja B 

PROVIDER: S-EPMC5540129 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature