Termination factor Rho: From the control of pervasive transcription to cell fate determination in Bacillus subtilis.

ABSTRACT: In eukaryotes, RNA species originating from pervasive transcription are regulators of various cellular processes, from the expression of individual genes to the control of cellular development and oncogenesis. In prokaryotes, the function of pervasive transcription and its output on cell physiology is still unknown. Most bacteria possess termination factor Rho, which represses pervasive, mostly antisense, transcription. Here, we investigate the biological significance of Rho-controlled transcription in the Gram-positive model bacterium Bacillus subtilis. Rho inactivation strongly affected gene expression in B. subtilis, as assessed by transcriptome and proteome analysis of a rho-null mutant during exponential growth in rich medium. Subsequent physiological analyses demonstrated that a cons

SUBMITTER: Bidnenko V 

PROVIDER: S-EPMC5540618 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature