Correcting nucleotide-specific biases in high-throughput sequencing data.

ABSTRACT:

Background

High-throughput sequence (HTS) data exhibit position-specific nucleotide biases that obscure the intended signal and reduce the effectiveness of these data for downstream analyses. These biases are particularly evident in HTS assays for identifying regulatory regions in DNA (DNase-seq, ChIP-seq, FAIRE-seq, ATAC-seq). Biases may result from many experiment-specific factors, including selectivity of DNA restriction enzymes and fragmentation method, as well as sequencing technology-specific factors, such as choice of adapters/primers and sample amplification methods.

Results

We present a novel method to detect and correct position-specific nucleotide biases in HTS short read data. Our method calculates read-specific weights based on aligned reads to correct the over-

SUBMITTER: Wang JR 

PROVIDER: S-EPMC5540620 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature