A novel plant-based-sea water culture media for in vitro cultivation and in situ recovery of the halophyte microbiome.

ABSTRACT: The plant-based-sea water culture medium is introduced to in vitro cultivation and in situ recovery of the microbiome of halophytes. The ice plant (Mesembryanthemum crystallinum) was used, in the form of juice and/or dehydrated plant powder packed in teabags, to supplement the natural sea water. The resulting culture medium enjoys the combinations of plant materials as rich source of nutrients and sea water exercising the required salt stress. As such without any supplements, the culture medium was sufficient and efficient to support very good in vitro growth of halotolerant bacteria. It was also capable to recover their in situ culturable populations in the phyllosphere, ecto-rhizosphere and endo-rhizosphere of halophytes prevailing in Lake Mariout, Egyp

SUBMITTER: Saleh MY 

PROVIDER: S-EPMC5540709 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature