Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging.

ABSTRACT: Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate. There is a real need for novel therapeutic approaches, but such advances require a detailed knowledge of C. albicans and its in vivo pathogenesis. Additionally, any novel antifungal drugs against C. albicans infections will need to be tested for their in vivo efficacy over time. Fungal pathogenesis and drug-mediated resolution studies can both be evaluated using non-invasive in vivo imaging technologies. In the work presented here, we used a codon-optimized firefly luciferase reporter system for detecting C.

SUBMITTER: Dorsaz S 

PROVIDER: S-EPMC5541039 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature