Single-Cell Growth Rates in Photoautotrophic Populations Measured by Stable Isotope Probing and Resonance Raman Microspectrometry.

ABSTRACT: A new method to measure growth rates of individual photoautotrophic cells by combining stable isotope probing (SIP) and single-cell resonance Raman microspectrometry is introduced. This report explores optimal experimental design and the theoretical underpinnings for quantitative responses of Raman spectra to cellular isotopic composition. Resonance Raman spectra of isogenic cultures of the cyanobacterium, Synechococcus sp., grown in ¹³C-bicarbonate revealed linear covariance between wavenumber (cm^-1) shifts in dominant carotenoid Raman peaks and a broad range of cellular ¹³C fractional isotopic abundance. Single-cell growth rates were calculated from spectra-derived isotopic content and empirical relationships. Growth rates among any 25 cells in a s

SUBMITTER: Taylor GT 

PROVIDER: S-EPMC5541042 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature