Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops.

ABSTRACT:

Background

Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. Expression of novel Cry proteins in food crops has however created apprehensions with respect to the safety aspects. To clarify this, premarket evaluation consisting of an array of analyses to evaluate the unintended effects is a prerequisite to provide safety assurance to the consumers. Additionally, series of bioinformatic tools as in silico aids are being used to evaluate the likely allergenic reaction of the proteins based on sequence and epitope similarity with known allergens.

Results

In the present study, chimeric Cry toxins developed through protein engineering were evalu

SUBMITTER: Rathinam M 

PROVIDER: S-EPMC5541426 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature