N-terminal α Dystroglycan (αDG-N): A Potential Serum Biomarker for Duchenne Muscular Dystrophy.

ABSTRACT:

Background

Duchenne Muscular Dystrophy (DMD) is a severe, progressive, neuromuscular disorder of childhood. While a number of serum factors have been identified as potential biomarkers of DMD, none, as yet, are proteins within the dystrophin-associated glycoprotein (DAG) complex.

Objective

We have developed an immobilized serum ELISA assay to measure the expression of a constitutively cleaved and secreted component of the DAG complex, the N-terminal domain of α dystroglycan (αDG-N), and assayed relative expression in serum from muscular dystrophy patients and normal controls.

Methods

ELISAs of immobilized patient or mouse serum and Western blots were used to assess αDG-N expression.

Results

Immobilization of diluted serum on ELISA plates was important for this

SUBMITTER: Crowe KE 

PROVIDER: S-EPMC5541672 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature