Cooperative RNA Folding under Cellular Conditions Arises From Both Tertiary Structure Stabilization and Secondary Structure Destabilization.

ABSTRACT: RNA folding has been studied extensively in vitro, typically under dilute solution conditions and abiologically high salt concentrations of 1 M Na⁺ or 10 mM Mg²⁺. The cellular environment is very different, with 20-40% crowding and only 10-40 mM Na⁺, 140 mM K⁺, and 0.5-2.0 mM Mg²⁺. As such, RNA structures and functions can be radically altered under cellular conditions. We previously reported that tRNA^phe secondary and tertiary structures unfold together in a cooperative two-state fashion under crowded in vivo-like ionic conditions, but in a noncooperative multistate fashion under dilute in vitro ionic conditions unless in nonphysiologically high concentrations of Mg²⁺. The mechanistic basis behind these effects r

SUBMITTER: Leamy KA 

PROVIDER: S-EPMC5542450 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature