Synthesis of Wavelength-Shifting Fluorescent DNA and RNA with Two Photostable Cyanine-Styryl Dyes as the Base Surrogate Pair.

ABSTRACT: Two nucleic acid building blocks were synthesized, consisting of two photostable green- and red-emitting cyanine-styryl dyes and (S)-3-amino-1,2-propanediol as a substitute for the ribofuranoside, and incorporated as base-pair surrogates by using automated phosphoramidte chemistry in the solid phase. The optical properties and, in particular, the energy-transfer properties were screened in a range of DNA duplexes, in which the "counter bases" of the two dyes were varied and the distance between the two dyes was enlarged to up to three intervening adenosine-thymidine pairs. The DNA duplex with the best optical properties and the best red/green emission ratio as the readout bore adenosine and thymidine opposite to the dyes, and the two dyes directly adjacent to each other as the base

SUBMITTER: Steinmeyer J 

PROVIDER: S-EPMC5542753 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature