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Visualizing adenosine-to-inosine RNA editing in single mammalian cells.

ABSTRACT: Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing levels vary.

SUBMITTER: Mellis IA 

PROVIDER: S-EPMC5542810 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Visualizing adenosine-to-inosine RNA editing in single mammalian cells.

Mellis Ian A IA   Gupte Rohit R   Raj Arjun A   Rouhanifard Sara H SH  

Nature methods 20170612 8

Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while G  ...[more]

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