In silico model of the human ClC-Kb chloride channel: pore mapping, biostructural pathology and drug screening.

ABSTRACT: The human ClC-Kb channel plays a key role in exporting chloride ions from the cytosol and is known to be involved in Bartter syndrome type 3 when its permeation capacity is decreased. The ClC-Kb channel has been recently proposed as a potential therapeutic target to treat hypertension. In order to gain new insights into the sequence-structure-function relationships of this channel, to investigate possible impacts of amino-acid substitutions, and to design novel inhibitors, we first built a structural model of the human ClC-Kb channel using comparative modeling strategies. We combined in silico and in vitro techniques to analyze amino acids involved in the chloride ion pathway as well as to rationalize the possible role of several clinically observed mutations leading to the Bartter syndrom

SUBMITTER: Louet M 

PROVIDER: S-EPMC5543074 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature