<i>AmoA</i>-Targeted Polymerase Chain Reaction Primers for the Specific Detection and Quantification of Comammox <i>Nitrospira</i> in the Environment.

ABSTRACT: Nitrification, the oxidation of ammonia via nitrite to nitrate, has always been considered to be catalyzed by the concerted activity of ammonia- and nitrite-oxidizing microorganisms. Only recently, complete ammonia oxidizers ("comammox"), which oxidize ammonia to nitrate on their own, were identified in the bacterial genus Nitrospira, previously assumed to contain only canonical nitrite oxidizers. Nitrospira are widespread in nature, but for assessments of the distribution and functional importance of comammox Nitrospira in ecosystems, cultivation-independent tools to distinguish comammox from strictly nitrite-oxidizing Nitrospira are required. Here we developed new PCR primer sets that specifically target the amoA genes coding for subunit A of the distin

SUBMITTER: Pjevac P 

PROVIDER: S-EPMC5543084 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature