Ex vivo pretreatment of human vessels with siRNA nanoparticles provides protein silencing in endothelial cells.

ABSTRACT: Human endothelial cells are initiators and targets of the rejection response. Pre-operative modification of endothelial cells by small interfering RNA transfection could shape the nature of the host response post-transplantation. Ablation of endothelial cell class II major histocompatibility complex molecules by small interfering RNA targeting of class II transactivator can reduce the capacity of human endothelial cells to recruit and activate alloreactive T cells. Here, we report the development of small interfering RNA-releasing poly(amine-co-ester) nanoparticles, distinguished by their high content of a hydrophobic lactone. We show that a single transfection of small interfering RNA targeting class II transactivator attenuates major histocompatibility complex class II expression on endo

SUBMITTER: Cui J 

PROVIDER: S-EPMC5543113 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature