Structural Basis for the Mechanism of ATP-Dependent Acetone Carboxylation.

ABSTRACT: Microorganisms use carboxylase enzymes to form new carbon-carbon bonds by introducing carbon dioxide gas (CO₂) or its hydrated form, bicarbonate (HCO₃^-), into target molecules. Acetone carboxylases (ACs) catalyze the conversion of substrates acetone and HCO₃^- to form the product acetoacetate. Many bicarbonate-incorporating carboxylases rely on the organic cofactor biotin for the activation of bicarbonate. ACs contain metal ions but not organic cofactors, and use ATP to activate substrates through phosphorylation. How the enzyme coordinates these phosphorylation events and new C-C bond formation in the absence of biotin has remained a mystery since these enzymes were discovered. The first structural rationale for acetone carboxylation i

SUBMITTER: Mus F 

PROVIDER: S-EPMC5543143 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature