Standardization of DNA Residual Quantification Method of Vero Cell Rabies Vaccine for Human Use.

ABSTRACT:

Objectives

Normalize the quantification of residual DNA from Vero cells in the rabies vaccine for use in human VAHV I, by quantitative PCR in real time and the design of primers that amplified, highly repetitive sequences of Cercopithecus aethiops and a constitutive gene according to sequences reported in the GenBank and quantifying the residual DNA in the vaccine VAHV I in three consecutive batches according to the standard set by the World Health Organization.

Methods

A real time quantitative method based on SYBR Green chemistry has been applied for the quantification of residual DNA (resDNA) using highly repetitive DNA (Alu) and a housekeeping gene (B-actin) as target sequences.

Results

The sensitivity achieved with this white sequence is within the reported

SUBMITTER: Palacios JDCA 

PROVIDER: S-EPMC5543725 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature