Small subunit ribosomal metabarcoding reveals extraordinary trypanosomatid diversity in Brazilian bats.

ABSTRACT:

Background

Bats are a highly successful, globally dispersed order of mammals that occupy a wide array of ecological niches. They are also intensely parasitized and implicated in multiple viral, bacterial and parasitic zoonoses. Trypanosomes are thought to be especially abundant and diverse in bats. In this study, we used 18S ribosomal RNA metabarcoding to probe bat trypanosome diversity in unprecedented detail.

Methodology/principal findings

Total DNA was extracted from the blood of 90 bat individuals (17 species) captured along Atlantic Forest fragments of Espírito Santo state, southeast Brazil. 18S ribosomal RNA was amplified by standard and/or nested PCR, then deep sequenced to recover and identify Operational Taxonomic Units (OTUs) for phylogenetic analysis. Blood sample

SUBMITTER: Dario MA 

PROVIDER: S-EPMC5544246 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature