Size and mobility of lipid domains tuned by geometrical constraints.

ABSTRACT: In the plasma membrane of eukaryotic cells, proteins and lipids are organized in clusters, the latter ones often called lipid domains or "lipid rafts." Recent findings highlight the dynamic nature of such domains and the key role of membrane geometry and spatial boundaries. In this study, we used porous substrates with different pore radii to address precisely the extent of the geometric constraint, permitting us to modulate and investigate the size and mobility of lipid domains in phase-separated continuous pore-spanning membranes (PSMs). Fluorescence video microscopy revealed two types of liquid-ordered (l_o) domains in the freestanding parts of the PSMs: (i) immobile domains that were attached to the pore rims and (ii) mobile, round-shaped l_o</

SUBMITTER: Schutte OM 

PROVIDER: S-EPMC5544307 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature