Effect of Methotrexate on an In Vitro Patient-Derived Model of Proliferative Vitreoretinopathy.

ABSTRACT:

Purpose

The purpose of this study was to develop a method for isolating, culturing, and characterizing cells from patient-derived membranes in proliferative vitreoretinopathy (PVR) to be used for drug testing.

Methods

PVR membranes were obtained from six patients with grade C PVR. Membrane fragments were analyzed by gross evaluation, fixed for immunohistologic studies to establish cell identity, or digested with collagenase II to obtain single cell suspensions for culture. PVR-derived primary cultures were used to examine the effects of methotrexate (MTX) on proliferation, migration, and cell death.

Results

Gross analysis of PVR membranes showed presence of pigmented cells, indicative of retinal pigment epithelial cells. Immunohistochemistry identified cells expressin

SUBMITTER: Amarnani D 

PROVIDER: S-EPMC5544356 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature