RNA SEQ Analysis Indicates that the AE3 Cl^-/HCO₃^- Exchanger Contributes to Active Transport-Mediated CO₂ Disposal in Heart.

ABSTRACT: Loss of the AE3 Cl^-/HCO₃^- exchanger (Slc4a3) in mice causes an impaired cardiac force-frequency response and heart failure under some conditions but the mechanisms are not known. To better understand the functions of AE3, we performed RNA Seq analysis of AE3-null and wild-type mouse hearts and evaluated the data with respect to three hypotheses (CO₂ disposal, facilitation of Na⁺-loading, and recovery from an alkaline load) that have been proposed for its physiological functions. Gene Ontology and PubMatrix analyses of differentially expressed genes revealed a hypoxia response and changes in vasodilation and angiogenesis genes that strongly support the CO₂ disposal hypothesis. Differential expression of energy metabolism gene

SUBMITTER: Vairamani K 

PROVIDER: S-EPMC5544674 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature