Conditional knock out of N-WASP in keratinocytes causes skin barrier defects and atopic dermatitis-like inflammation.

ABSTRACT: Neural-Wiskott Aldrich Syndrome Protein (N-WASP) is expressed ubiquitously and regulates actin cytoskeleton remodeling. In order to characterize the role of N-WASP in epidermal homeostasis and cutaneous biology, we generated conditional N-WASP knockout mouse using CK14-cre (cytokeratin 14) to ablate expression of N-WASP in keratinocytes. N-WASP^K14KO (N-WASP ^fl/fl ; CK14-Cre) mice were born following Mendelian genetics suggesting that N-WASP expression in keratinocytes is not essential during embryogenesis. N-WASP^K14KO mice exhibited stunted growth, alopecia, dry and wrinkled skin. The dry skin in N-WASP^K14KO mice is probably due to increased transepidermal water loss (TEWL) caused by barrier function defects as revealed by dye penetration assay.

SUBMITTER: Kalailingam P 

PROVIDER: S-EPMC5544743 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature