A novel secretion and online-cleavage strategy for production of cecropin A in Escherichia coli.

ABSTRACT: Antimicrobial peptides, promising antibiotic candidates, are attracting increasing research attention. Current methods for production of antimicrobial peptides are chemical synthesis, intracellular fusion expression, or direct separation and purification from natural sources. However, all these methods are costly, operation-complicated and low efficiency. Here, we report a new strategy for extracellular secretion and online-cleavage of antimicrobial peptides on the surface of Escherichia coli, which is cost-effective, simple and does not require complex procedures like cell disruption and protein purification. Analysis by transmission electron microscopy and semi-denaturing detergent agarose gel electrophoresis indicated that fusion proteins contain cecropin A peptides can successfully be

SUBMITTER: Wang M 

PROVIDER: S-EPMC5544755 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature