Comparative Sequence Analysis of Multidrug-Resistant IncA/C Plasmids from Salmonella enterica.

ABSTRACT: Determinants of multidrug resistance (MDR) are often encoded on mobile elements, such as plasmids, transposons, and integrons, which have the potential to transfer among foodborne pathogens, as well as to other virulent pathogens, increasing the threats these traits pose to human and veterinary health. Our understanding of MDR among Salmonella has been limited by the lack of closed plasmid genomes for comparisons across resistance phenotypes, due to difficulties in effectively separating the DNA of these high-molecular weight, low-copy-number plasmids from chromosomal DNA. To resolve this problem, we demonstrate an efficient protocol for isolating, sequencing and closing IncA/C plasmids from Salmonella sp. using single molecule real-time sequencing on a Pacific Biosciences (P

SUBMITTER: Hoffmann M 

PROVIDER: S-EPMC5545573 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature