Transcriptome profiling of tobacco (Nicotiana tabacum) pollen and pollen tubes.

ABSTRACT:

Background

Pollen tube growth is essential for plant reproduction and represents a widely employed model to investigate polarized cell expansion, a process important for plant morphogenesis and development. Cellular and regulatory mechanisms underlying pollen tube elongation are under intense investigation, which stands to greatly benefit from a comprehensive understanding of global gene expression profiles in pollen and pollen tubes. Here, RNA sequencing technology was applied to de novo assemble a Nicotiana tabacum male gametophytic transcriptome and to compare transcriptome profiles at two different stages of gametophyte development: mature pollen grains (MPG) and pollen tubes grown for six hours in vitro (PT6).

Results

De novo assembly of data obtained by 454 sequencing

SUBMITTER: Conze LL 

PROVIDER: S-EPMC5545845 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature