Effects of Fungicides on Rat's Neurosteroid Synthetic Enzymes.

ABSTRACT: Exposure to environmental endocrine disruptors may interfere with nervous system's activity. Fungicides such as tebuconazole, triadimefon, and vinclozolin have antifungal activities and are used to prevent fungal infections in agricultural plants. In the present study, we studied effects of tebuconazole, triadimefon, and vinclozolin on rat's neurosteroidogenic 5α-reductase 1 (5α-Red1), 3α-hydroxysteroid dehydrogenase (3α-HSD), and retinol dehydrogenase 2 (RDH2). Rat's 5α-Red1, 3α-HSD, and RDH2 were cloned and expressed in COS-1 cells, and effects of these fungicides on them were measured. Tebuconazole and triadimefon competitively inhibited 5α-Red1, with IC₅₀ values of 8.670 ± 0.771 × 10^-6 M and 17.390 ± 0.079 × 10

SUBMITTER: Shen X 

PROVIDER: S-EPMC5546122 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature