Reconstructing the regulatory circuit of cell fate determination in yeast mating response.

ABSTRACT: Massive technological advances enabled high-throughput measurements of proteomic changes in biological processes. However, retrieving biological insights from large-scale protein dynamics data remains a challenging task. Here we used the mating differentiation in yeast Saccharomyces cerevisiae as a model and developed integrated experimental and computational approaches to analyze the proteomic dynamics during the process of cell fate determination. When exposed to a high dose of mating pheromone, the yeast cell undergoes growth arrest and forms a shmoo-like morphology; however, at intermediate doses, chemotropic elongated growth is initialized. To understand the gene regulatory networks that control this differentiation switch, we employed a high-throughput microfluidic imaging system tha

SUBMITTER: Shao B 

PROVIDER: S-EPMC5546706 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature