Long-term safety from transmission of porcine endogenous retrovirus after pig-to-non-human primate corneal transplantation.

ABSTRACT:

Background

The risk of xenozoonosis mainly by porcine endogenous retrovirus (PERV) has been considered as one of the main hurdles in xenotransplantation and therefore should be elucidated prior to the clinical use of porcine corneal grafts. Accordingly, an investigation was performed to analyze the infectivity of PERVs from porcine keratocytes to human cells, and the long-term risk of transmission of PERVs was determined using pig-to-non-human primate (NHP) corneal transplantation models.

Methods

The infectivity of PERVs from the SNU miniature pig keratocytes was investigated by coculture with a human embryonic kidney cell line. Twenty-two rhesus macaques underwent xenocorneal transplantation as follows: (i) group 1 (n=4): anterior lamellar keratoplasty (LKP) with freshly pr

SUBMITTER: Choi HJ 

PROVIDER: S-EPMC5546926 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature