Characterization of rapid extraction protocols for high-throughput metabolomics.

ABSTRACT:

Rationale

In the last five years, high-throughput metabolomics has significantly advanced scientific research and holds the potential to promote strides in the fields of clinical metabolomics and personalized medicine. While innovations in the field of flow-injection mass spectrometry and three-minute metabolomics methods now allow investigators to process hundreds to thousands of samples per day, time-sensitive clinical applications, particularly in the emergency department, are limited by a lack of rapid extraction methods.

Methods

Here we characterized the efficacy of fast liquid-liquid extractions for characterization of hydrophilic compounds through ultra-high-pressure liquid chromatography/mass spectrometry. Internal stable-isotope-labeled standards were used to quanti

SUBMITTER: Gehrke S 

PROVIDER: S-EPMC5547002 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature