The rational design of a 'type 88' genetically stable peptide display vector in the filamentous bacteriophage fd.

ABSTRACT: Filamentous bacteriophages are particularly efficient for the expression and display of combinatorial random peptides. Two phage proteins are often employed for peptide display: the infectivity protein, PIII, and the major coat protein, PVIII. The use of PVIII typically requires the expression of two pVIII genes: the wild-type and the recombinant pVIII gene, to generate mosaic phages. 'Type 88' vectors contain two pVIII genes in one phage genome. In this study a novel 'type 88' expression vector has been rationally designed and constructed. Two factors were taken into account: the insertion site and the genetic stability of the second pVIII gene. It was found that selective deletion of recombinant genes was encountered when inserts were cloned into either of the two non-coding regions of t

SUBMITTER: Enshell-Seijffers D 

PROVIDER: S-EPMC55471 | biostudies-literature | 2001 May

REPOSITORIES: biostudies-literature