Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange.

ABSTRACT: High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated mat

SUBMITTER: Browning KL 

PROVIDER: S-EPMC5547137 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature