Molecular identification of Nocardia species using the sodA gene: Identificacion molecular de especies de Nocardia utilizando el gen sodA.

ABSTRACT: Currently for bacterial identification and classification the rrs gene encoding 16S rRNA is used as a reference method for the analysis of strains of the genus Nocardia. However, it does not have enough polymorphism to differentiate them at the species level. This fact makes it necessary to search for molecular targets that can provide better identification. The sodA gene (encoding the enzyme superoxide dismutase) has had good results in identifying species of other Actinomycetes. In this study the sodA gene is proposed for the identification and differentiation at the species level of the genus Nocardia. We used 41 type species of various collections; a 386 bp fragment of the sodA gene was amplified and sequenced, and a phylogenetic analysi

SUBMITTER: Sanchez-Herrera K 

PROVIDER: S-EPMC5547243 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature