Conclusive evidence for hexasomic inheritance in chrysanthemum based on analysis of a 183 k SNP array.

ABSTRACT:

Background

Cultivated chrysanthemum is an outcrossing hexaploid (2n = 6× = 54) with a disputed mode of inheritance. In this paper, we present a single nucleotide polymorphism (SNP) selection pipeline that was used to design an Affymetrix Axiom array with 183 k SNPs from RNA sequencing data (1). With this array, we genotyped four bi-parental populations (with sizes of 405, 53, 76 and 37 offspring plants respectively), and a cultivar panel of 63 genotypes. Further, we present a method for dosage scoring in hexaploids from signal intensities of the array based on mixture models (2) and validation of selection steps in the SNP selection pipeline (3). The resulting genotypic data is used to draw conclusions on the mode of inheritance in chrysanthemum (4), and to make an inference on all

SUBMITTER: van Geest G 

PROVIDER: S-EPMC5547472 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature