Large scale genomic reorganization of topological domains at the HoxD locus.

ABSTRACT:

Background

The transcriptional activation of HoxD genes during mammalian limb development involves dynamic interactions with two topologically associating domains (TADs) flanking the HoxD cluster. In particular, the activation of the most posterior HoxD genes in developing digits is controlled by regulatory elements located in the centromeric TAD (C-DOM) through long-range contacts.

Results

To assess the structure-function relationships underlying such interactions, we measured compaction levels and TAD discreteness using a combination of chromosome conformation capture (4C-seq) and DNA FISH. We assessed the robustness of the TAD architecture by using a series of genomic deletions and inversions that impact the integrity of this chromatin domain and that remodel long-range c

SUBMITTER: Fabre PJ 

PROVIDER: S-EPMC5547506 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature