Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

ABSTRACT: Emerging studies have linked the ribosome to more selective control of gene regulation. However, an outstanding question is whether ribosome heterogeneity at the level of core ribosomal proteins (RPs) exists and enables ribosomes to preferentially translate specific mRNAs genome-wide. Here, we measured the absolute abundance of RPs in translating ribosomes and profiled transcripts that are enriched or depleted from select subsets of ribosomes within embryonic stem cells. We find that heterogeneity in RP composition endows ribosomes with differential selectivity for translating subpools of transcripts, including those controlling metabolism, cell cycle, and development. As an example, mRNAs enriched in binding to RPL10A/uL1-containing ribosomes are shown to require RPL10A/uL1 for their effi

SUBMITTER: Shi Z 

PROVIDER: S-EPMC5548184 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature