Fluorescent indicators for simultaneous reporting of all four cell cycle phases.

ABSTRACT: A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.

SUBMITTER: Bajar BT 

PROVIDER: S-EPMC5548384 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature