Dynamics of Crowding-Induced Mixing in Phase Separated Lipid Bilayers.

ABSTRACT: We use fluorescence microscopy to examine the dynamics of the crowding-induced mixing transition of liquid ordered (L_o)-liquid disordered (L_d) phase separated lipid bilayers when the following particles of increasing size bind to either the L_o or L_d phase: Ubiquitin, green fluorescent protein (GFP), and nanolipoprotein particles (NLPs) of two diameters. These proteinaceous particles contained histidine-tags, which were phase targeted by binding to iminodiacetic acid (IDA) head groups, via a Cu²⁺ chelating mechanism, of lipids that specifically partition into either the L_o phase or L_d phase. The degree of steric pressure was controlled by varying the size of the bound particle (10-240 kDa) and the amount of binding

SUBMITTER: Zeno WF 

PROVIDER: S-EPMC5548394 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature