Differentiation impairs Bach1 dependent HO-1 activation and increases sensitivity to oxidative stress in SH-SY5Y neuroblastoma cells.

ABSTRACT: Neuronal adaptation to oxidative stress is crucially important in order to prevent degenerative diseases. The role played by the Nrf2/HO-1 system in favoring cell survival of neuroblastoma (NB) cells exposed to hydrogen peroxide (H₂O₂) has been investigated using undifferentiated or all-trans retinoic acid (ATRA) differentiated SH-SY5Y cells. While undifferentiated cells were basically resistant to the oxidative stimulus, ATRA treatment progressively decreased cell viability in response to H₂O₂. HO-1 silencing decreased undifferentiated cell viability when exposed to H₂O₂, proving the role of HO-1 in cell survival. Conversely, ATRA differentiated cells exposed to H₂O₂ showed a significantly lower induct

SUBMITTER: Piras S 

PROVIDER: S-EPMC5548785 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature