Conformational modulation of influenza virus hemagglutinin: characterization and in vivo efficacy of monomeric form.

ABSTRACT: Mutational changes that mostly occur at the head region of hemagglutinin (HA) lead to the emergence of new epidemic influenza viruses, whereas HA antigens have been modified to generate broadly neutralizing antibodies toward highly conserved epitopes in the HA stem. Interestingly, a recent analysis of serum antibody repertoires showed that broadly neutralizing antibodies bind to HA monomer at a conserved region occluded at the intermonomer interface of HA trimer and confer protection in animal models. We showed previously that the recombinant HA ectodomain from a pandemic strain A/Korea/01/2009 was monomeric in solution and crystal structure. In order to examine the potential antigenicity of a monomeric form, we designed HA monomer that incorporates mutations to destabilize trimer conforma

SUBMITTER: Seok JH 

PROVIDER: S-EPMC5548806 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature