Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples.

ABSTRACT:

Background

Sequence-specific PCR is the most common approach for virus identification in diagnostic laboratories. However, as specific PCR only detects pre-defined targets, novel virus strains or viruses not included in routine test panels will be missed. Recently, advances in high-throughput sequencing allow for virus-sequence-independent identification of entire virus populations in clinical samples, yet standardized protocols are needed to allow broad application in clinical diagnostics. Here, we describe a comprehensive sample preparation protocol for high-throughput metagenomic virus sequencing using random amplification of total nucleic acids from clinical samples.

Results

In order to optimize metagenomic sequencing for application in virus diagnostics, we tested diffe

SUBMITTER: Lewandowska DW 

PROVIDER: S-EPMC5549297 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature