Catalytic and functional aspects of different isozymes of glycolate oxidase in rice.

ABSTRACT:

Background

Glycolate oxidase (GLO) is a key enzyme for photorespiration in plants. There are four GLO genes encoding and forming different isozymes in rice, but their functional differences are not well understood. In this study, enzymatic and physiological characteristics of the GLO isozymes were comparatively analyzed.

Results

When expressed heterologously in yeast, GLO1, GLO4 and GLO1 + 4 showed the highest activities and lowest K _m for glycolate as substrate, whereas GLO3 displayed high activities and affinities for both glycolate and L-lactate, and GLO5 was catalytically inactive with all substrates tested. To further reveal the physiological role of each GLO isozyme in plants, various GLO genetically modified rice lines were generated and functionally analyz

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC5549332 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature