Intrinsically Disordered Stress Protein COR15A Resides at the Membrane Surface during Dehydration.

ABSTRACT: Plants from temperate climate zones are able to increase their freezing tolerance during exposure to low, above-zero temperatures in a process termed cold acclimation. During this process, several cold-regulated (COR) proteins are accumulated in the cells. One of them is COR15A, a small, intrinsically disordered protein that contributes to leaf freezing tolerance by stabilizing cellular membranes. The isolated protein folds into amphipathic α-helices in response to increased crowding conditions, such as high concentrations of glycerol. Although there is evidence for direct COR15A-membrane interactions, the orientation and depth of protein insertion were unknown. In addition, although folding due to high osmolyte concentrations had been established, the folding response of the protein under

SUBMITTER: Bremer A 

PROVIDER: S-EPMC5549682 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature