MALDI-TOF MS typing enables the classification of brewing yeasts of the genus Saccharomyces to major beer styles.

ABSTRACT: Brewing yeasts of the genus Saccharomyces are either available from yeast distributor centers or from breweries employing their own "in-house strains". During the last years, the classification and characterization of yeasts of the genus Saccharomyces was achieved by using biochemical and DNA-based methods. The current lack of fast, cost-effective and simple methods to classify brewing yeasts to a beer type, may be closed by Matrix Assisted Laser Desorption/Ionization-Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) upon establishment of a database based on sub-proteome spectra from reference strains of brewing yeasts. In this study an extendable "brewing yeast" spectra database was established including 52 brewing yeast strains of the most important types of bottom- and top-fermenting stra

SUBMITTER: Lauterbach A 

PROVIDER: S-EPMC5549903 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature