The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling.

ABSTRACT: Regenerating tissue must initiate the signaling that drives regenerative growth, and sustain that signaling long enough for regeneration to complete. How these key signals are sustained is unclear. To gain a comprehensive view of the changes in gene expression that occur during regeneration, we performed whole-genome mRNAseq of actively regenerating tissue from damaged Drosophila wing imaginal discs. We used genetic tools to ablate the wing primordium to induce regeneration, and carried out transcriptional profiling of the regeneration blastema by fluorescently labeling and sorting the blastema cells, thus identifying differentially expressed genes. Importantly, by using genetic mutants of several of these differentially expressed genes we have confirmed that they have roles in regeneratio

SUBMITTER: Khan SJ 

PROVIDER: S-EPMC5550008 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature